To use all functions of this page, please activate cookies in your browser.
With an accout for my.chemeurope.com you can always see everything at a glance – and you can configure your own website and individual newsletter.
- My watch list
- My saved searches
- My saved topics
- My newsletter
Additional recommended knowledge
In general, different NPF-containing proteins are found as binding partners for the different EH domain-containing proteins (2). A major question is how the selection is made. The human genome encodes 17 to 19 EH domains contained in 11 proteins that can be divided into 5 functional groups based on homology and domain structure: 1, Eps15 and the related protein Eps15R, which contain 3 EH domains each; 2, Intersectin, and its isoform intersectin-2, which contain 2 EH domains each; 3, Reps1 (and the related POB1/Reps2) containing 1 or 2 EH domains each; 4, gamma-synergin; and 5, the 4 EHD proteins, which contain 1 EH domain each. There are multiple (perhaps 30) potential binding proteins containing multiple NPF motifs.
EH domain containing proteins are generally involved in vesicle transport. They have been implicated in several signalling pathways, including signalling from the Ras-like protein, Ral (12). More recently, they have been shown to be important for the expression of glucose transporters in response to insulin (13).
1. Ikura, M. (1996) Calcium binding and conformational response in EF-hand proteins Trends in Biochem. Sci. 21, 14-17 2. Confalonieri, S., & Di Fiore, P. P. (2002) The Eps15 homology (EH) domain FEBS Lett 513, 24-29. 3. Chen, H., Fre, S., Slepnev, V. I., Capua, M. R., Takei, K., Butler, M. H., Di Fiore, P. P., & De Camilli, P. (1998) Epsin is an EH-domain-binding protein implicated in clathrin-mediated endocytosis Nature 394, 793-797. 4. Morinaka, K., Koyama, S., Nakashima, S., Hinoi, T., Okawa, K., Iwamatsu, A., & Kikuchi, A. (1999) Epsin binds to the EH domain of POB1 and regulates receptor-mediated endocytosis Oncogene 18, 5915-5922 5. McPherson, P. S., de Heuvel, E., Phillie, J., Wang, W., Sengar, A., & Egan, S. (1998) EH domain-dependent interactions between Eps15 and clathrin-coated vesicle protein p95 Biochem Biophys Res Commun 244, 701-705. 6. de Beer, T., Carter, R. E., Lobel-Rice, K. E., Sorkin, A., & Overduin, M. (1998) Structure and asn-pro-Phe binding pocket of the eps15 homology domain Science 281, 1357-1360 7. Koshiba, S., Kigawa, T., Iwahara, J., Kikuchi, A., & Yokoyama, S. (1999) Solution structure of the Eps15 homology domain of a human POB1 (partner of RalBP1) FEBS Lett 442, 138-142 8. Whitehead, B., Tessari, M., Carotenuto, A., van Bergen en Henegouwen, P. M., & Vuister, G. W. (1999) The EH1 domain of Eps15 is structurally classified as a member of the S100 subclass of EF-hand-containing proteins Biochemistry 38, 11271-11277 9. Enmon, J. L., de Beer, T., & Overduin, M. (2000) Solution Structure of Eps15's Third EH Domain Reveals Coincident Phe- Trp and Asn-Pro-Phe Binding Sites Biochemistry 39, 4309-4319 10. Kim, S., Cullis, D. N., Feig, L. A., & Baleja, J. D. (2001) Solution Structure of the Reps1 EH Domain and Characterization of Its Binding to NPF Target Sequences Biochemistry 40, 6776-6785 11. de Beer, T., Hoofnagle, A. N., Enmon, J. L., Bowers, R. C., Yamabhai, M., Kay, B. K., & Overduin, M. (2000) Molecular mechanism of NPF recognition by EH domains Nat Struct Biol 7, 1018-1022 12. Cullis, D. N., B. Philip, J. D. Baleja and L. A. Feig (2002). Rab11-FIP2, an Adaptor Protein Connecting Cellular Components Involved in Internalization and Recycling of Epidermal Growth Factor Receptors. J Biol Chem 277, 49158-66. 13. Guilherme, A., N. A. Soriano, P. S. Furcinitti and M. P. Czech (2004). Role of EHD1 and EHBP1 in Perinuclear Sorting and Insulin-regulated GLUT4 Recycling in 3T3-L1 Adipocytes. J Biol Chem 279, 40062-75.
|This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "EH_domain". A list of authors is available in Wikipedia.|