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Hygromycin B



Hygromycin B
Systematic (IUPAC) name
O-6-Amino-6-deoxy-L-glycero- D-galacto- heptopyranosylidene- (1-2-3)-O-beta-D-talopyranosyl (1-5) -2-deoxy-N3-methyl-D-streptamine
Identifiers
CAS number 31282-04-9
ATC code  ?
PubChem 35766
Chemical data
Formula C20H37N3O13 
Mol. mass 527.53 g/mol (563.5 with HCl)
Pharmacokinetic data
Bioavailability  ?
Metabolism  ?
Half life  ?
Excretion  ?
Therapeutic considerations
Pregnancy cat.

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Legal status
Routes  ?

Hygromycin B, is an antibiotic produced by the bacterium Streptomyces hygroscopicus. It is an aminoglycoside that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis[1].

Additional recommended knowledge

History

Hygromycin B was originally developed in the 1950's for use with animals and is still added into swine and chicken feed as an anthelmintic or anti-worming agent (product name: Hygromix). Hygromycin B is produced by Streptomyces hygroscopicus, a bacterium isolated in 1953 from a soil sample. Resistance genes were discovered in the early 1980's.[2][3]

Use in research

In the laboratory it is used for the selection and maintenance of prokaryotic and eukaryotic cells that contain the hygromycin resistance gene. The resistance gene is a kinase that inactivates hygromycin B through phosphorylation.[4] Since the discovery of hygromycin-resistance genes, hygromycin B has become a standard selection antibiotic in gene transfer experiments in many prokaryotic and eukaryotic cells.

References

  1. ^ McGuire, Pettinger (1953), " ", Antibiot. Chemother. 3: 1268-1278
  2. ^ Davies, Gritz (1983), " ", Gene 25: 179-88
  3. ^ Burgett, Kaster (1983), " ", Nucleic Acids Res. 11: 6895-911
  4. ^ Rao RN, Allen NE, Hobbs JN, Alborn WE, Kirst HA, Paschal JW (1983). "Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli". Antimicrobial Agents and Chemotherapy 24 (5): 689-95. PMID 6318654.
 
This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Hygromycin_B". A list of authors is available in Wikipedia.
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