Light Green SF yellowish

  Light Green SF yellowish, or Light Green, Acid Green, Lissamine green SF, Acid Green 5', Food Green 2, FD&C Green no. 2, Green No. 205, Acid Brilliant Green 5, Pencil Green SF, or C.I. 42095, is a green triarylmethane dye. It is used in histology for staining collagen; for that purpose it is a standard dye in North America. In Masson's trichrome it is used as a counterstain to acid fuchsin. It is a critical component of Papanicolaou stains together with eosin Y and bismarck brown Y. It usually comes as a disodium salt. Its maximum absorption is at 630 (422) nm.

Additional recommended knowledge

Essential Laboratory Skills Guide

Daily Visual Balance Check

Daily Sensitivity Test

The dye is not very durable -- it has a tendency to fade. When fading is to be avoided, it is replaced with Fast Green FCF, which also has more brilliant color. Fast Green FCF can also substitute Light Green SF yellowish in many other procedures.

Its CAS number is [5141-20-8] and its SMILES structure is CCN(Cc1cccc(OS([O-])=O)c1)c2ccc(cc2)/C(c3ccc(OS([O-])=O)cc3)=C4/C=C\C(C=C4)=[N+](\CC)Cc5cccc(OS([O-])=O)c5.