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RNA spike-in

An RNA spike-in is an RNA transcript used to calibrate measurements in a DNA microarray experiment. Each spike-in is designed to hybridize with a specific control probe on the target array. Manufacturers of commercially available microarrays typically offer companion RNA spike-ins "kits".

Known amounts of RNA spike-ins are mixed with the experiment sample during preparation. Subsequently the measured degree of hybridization between the spike-ins and the control probes is used to normalize the hybridization measurements of the sample RNA.

This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "RNA_spike-in". A list of authors is available in Wikipedia.
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