To use all functions of this page, please activate cookies in your browser.
With an accout for my.chemeurope.com you can always see everything at a glance – and you can configure your own website and individual newsletter.
- My watch list
- My saved searches
- My saved topics
- My newsletter
Zinc finger protein transcription factor
Zinc finger protein transcription factors or ZFP-TFs, consisting of activators and repressors are transcription factors composed of a zinc finger protein domain (ZFP) and any of a variety of transcription-factor effector-domains which exert their modulatory effect in the vicinity of any sequence to which the ZFP domain binds.
Additional recommended knowledge
Zinc finger protein transcription factors can be encoded by genes small enough to fit a number of such genes into a single vector, allowing the medical intervention and control of expression of multiple genes and the initiation of an elaborate cascade of events. In this respect, it is also possible to target a sequence which is common to multiple (usually functionally related) genes in order to control the transcription of all these genes with a single transcription factor. Alternatively, it is possible to target a family of related genes by targeting and modulating the expression of the endogenous transcription factor(s) which control(s) them. They also have the advantage that the targeted sequence need not be symmetrical unlike with most other DNA-binding motifs based on natural transcription factors which bind as dimers.
By targeting the ZFP towards a specific DNA sequence and attaching the necessary effector domain, it is possible to downregulate or upregulate the expression of the gene(s) in question while using the same DNA-binding domain. The expression of a gene can also be downregulated by blocking elongation by RNA polymerase (without the need for an effector domain) in the coding region or alternatively, RNA itself can also be targeted. Besides the obvious development of tools for the research of gene function, engineered ZFP-TFs have enormous therapeutic potential including correction of abnormal gene expression profiles (e.g. erbB-2 overexpression in human adenocarcinomas and anti-retrovirals (e.g. HIV-1).
|This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Zinc_finger_protein_transcription_factor". A list of authors is available in Wikipedia.|