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Amido black

Amido black
IUPAC name 4-Amino-5-hydroxy-3-[(4-nitrophenyl)azo]-6- -(phenylazo)-2,7-Naphthalene disulfonic acid, disodium salt
CAS number 1064-48-8
PubChem 9566044
SMILES OC1=C(C(N)=C(/N=N/C4=CC=C([N+]([O-])=O) C=C4)C(S(=O)(O[Na])=O)=C3)C3=CC(S(=O) (O[Na])=O)=C1/N=N/C2=CC=CC=C2
Molecular formula C22H14N6Na2O9S2
Molar mass 616.491
Appearance Dark red to black
Solubility in water ~ 30 g/l at 20 °C
NFPA 704
R-phrases R20, R21, R33
S-phrases S28A, S37, S45
Except where noted otherwise, data are given for
materials in their standard state
(at 25 °C, 100 kPa)

Infobox disclaimer and references

Amido black 10B, also called Amidoschwarz, Naphthol blue black, Acid Black 1, Acidal Black 10B , Acidal Navy Blue 3BR, Naphthalene Black 10B, Buffalo Black NBR and C.I. 20470, is an amino acid staining diazo dye used in biochemical research to stain for total protein on transferred membrane blots. It is also used in criminal investigations to detect blood present with latent fingerprints. It stains the proteins in blood a blue-black color. Amido Black can be either methanol or water based as it readily dissolves in both. With picric acid, in a van Gieson procedure, it can be used to stain collagen and reticulin.

This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Amido_black". A list of authors is available in Wikipedia.
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