EcoRII

EcoRII is an restriction enzyme obtained of Escherichia coli, a Gram-negative bacteria. This enzyme recognizes asymmetric, pseudopalindromic dsDNA sequences, and cuts generating sticky ends.

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Cut diagram:

5' NNCCWGGNN
3' NNGGWCCNN 

5' NN  CCWGGNN
3' NNGGWCC  NN

W = A or T

References

• REBASE - The Restriction Enzyme Database
• Griffiths, J .F. A. et al. (2002): Genética. McGraw-Hill Interamericana, ISBN 84-486-0368-0.