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Fast protein liquid chromatography



Fast performance liquid chromatography
Acronym FPLC
Classification Chromatography
Other Techniques
Related High performance liquid chromatography
Affinity chromatography

Fast Protein Liquid Chromatography, usually referred to as FPLC, is a form of column chromatography used to separate or purify proteins from complex mixtures by using Ion Chromatography. It is very commonly used in biochemistry and enzymology. Columns used with an FPLC can separate macromolecules based on size, charge distribution, hydrophobicity, or biorecognition (as with affinity chromatography).

Additional recommended knowledge

Trademark

FPLC is a trademark of GE Healthcare, formerly Amersham-Pharmacia Biotech and Pharmacia Biotech.

Typical columns

Typical columns used for protein purification include:

  • Ion exchange chromatography, cation or anion — separate proteins based on surface-charges
  • Size exclusion chromatography, also known as Gel filtration chromatography, Gel permeation chromatography or desalting — separate proteins based on size.
  • Reversed phase or hydrophobic interaction — separates based on hydrophobicity which depends heavily on surface area
  • Affinity chromatography — columns which purify based on ligand affinity (e.g. a His6-tagged protein purified on a nickel column).

See also

 
This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Fast_protein_liquid_chromatography". A list of authors is available in Wikipedia.
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