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Fast protein liquid chromatography
Fast Protein Liquid Chromatography, usually referred to as FPLC, is a form of column chromatography used to separate or purify proteins from complex mixtures by using Ion Chromatography. It is very commonly used in biochemistry and enzymology. Columns used with an FPLC can separate macromolecules based on size, charge distribution, hydrophobicity, or biorecognition (as with affinity chromatography).
Additional recommended knowledge
FPLC is a trademark of GE Healthcare, formerly Amersham-Pharmacia Biotech and Pharmacia Biotech.
Typical columns used for protein purification include:
|This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Fast_protein_liquid_chromatography". A list of authors is available in Wikipedia.