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CAS number 113694-64-7
Molecular formula C29H22N3O145-
Molar mass 636.50 g/mol
Except where noted otherwise, data are given for
materials in their standard state
(at 25 °C, 100 kPa)

Infobox disclaimer and references

Fura-2 is a ratiometric fluorescent dye which binds to free intracellular calcium.[1] It was the first highly used dye for calcium imaging and remains very popular. Fura-2 is excited at 340 nm and 380 nm of light, and the ratio of the emissions at those wavelengths is directly correlated to the amount of intracellular calcium. The use of the ratio allows freedom from a myriad of confounding factors, such as dye concentration, autofluorescent background, focus drift and ambient light, making Fura-2 one of the most preferred tools to quantify calcium levels. More recently, genetically encoded calcium indicators based on spectral variants of the green fluorescent protein, such as Cameleons[2], have supplemented the use of Fura-2 and other small molecule dyes for calcium imaging.

See also

  • Fura-2AM, a membrane-permeable derivative of Fura-2


  1. ^ Grynkiewicz, G., Poenie, M., and Tsien, R.Y. (1985). "A new generation of CA2+ indicators with greatly improved fluorescence properties*". J.Biol.Chem. 260: 3440-3450.
  2. ^ Miyawaki A, Llopis J, Heim R, et al (1997). "Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin". Nature 388 (6645): 882–7. doi:10.1038/42264. PMID 9278050.
This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Fura-2". A list of authors is available in Wikipedia.
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