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Human serum albumin
Human serum albumin is the most abundant protein in human blood plasma. It is produced in the liver. Albumin comprises about half of the blood serum protein. It is soluble and monomeric.
The gene for albumin is located on chromosome 4 and mutations in this gene can result in various anomalous proteins. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain.
Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
The reference range for albumin concentrations in blood is 30 to 50 g/L. It has a serum half-life of approximately 20 days. It has a molecular mass of 67 kDa.
Additional recommended knowledge
Functions of albumin
Low blood albumin levels (hypoalbuminemia) can be caused by:
Typically is a sign of severe dehydration.
Glycation (Glycosylation) of Serum Albumin
It has been known for a long time that human blood proteins like hemoglobin  and serum albumin  may undergo a slow non-enzymatic glycation, mainly by formation of a Schiff base between ε-amino groups of lysine (and sometimes arginine) residues and glucose molecules in blood (Millard reaction). This reaction can be inhibited in the presence of antioxidant agents . Although this reaction may happen normally  , elevated glycoalbumin is observed in diabetes mellitus .
Glycation has the potential to alter the biological structure and function of the serum albumin protein . Moreover, the glycation finally can result in the formation of Advanced Glycosylation End Products (AGE), which result in abnormal biological effects. Accumulation of AGEs leads to tissue damage via alteration of the structures and functions of tissue proteins, stimulation of cellular responses, through receptors specific for AGE-proteins, and via generation of reactive oxygen intermediates. AGEs also react with DNA, thus causing mutations and DNA transposition. Thermal processing of proteins and carbohydrates brings major changes in allergenicity. AGEs are antigenic and represent many of the important neoantigens found in cooked or stored foods . They also interfere with the normal product of nitric oxide in cells .
Although there are several lysine and arginine residues in the serum albumin structure, very few of them can take part in the glycation reaction . It is not clear exactly why only these residues are glycated in serum albumin .
Testing for albumin loss via the kidneys
In the healthy kidney, albumin's size and negative electric charge exclude it from excretion in the glomerulus. This is not always the case, as in some diseases including diabetic nephropathy, a major complication of uncontrolled diabetes where proteins can cross the glomerulus. The lost albumin can be detected by a simple urine test . Depending on the amount of albumin lost, a patient may have normal renal function, microalbuminuria, or albuminuria.
Amino Acid Sequence
The approximate sequence of human serum albumin is:
MKWVTFISLL FLFSSAYSRG VFRRDAHKSE VAHRFKDLGE ENFKALVLIA FAQYLQQCPF EDHVKLVNEV TEFAKTCVAD ESAENCDKSL HTLFGDKLCT VATLRETYGE MADCCAKQEP ERNECFLQHK DDNPNLPRLV RPEVDVMCTA FHDNEETFLK KYLYEIARRH PYFYAPELLF FAKRYKAAFT ECCQAADKAA CLLPKLDELR DEGKASSAKQ RLKCASLQKF GERAFKAWAV ARLSQRFPKA EFAEVSKLVT DLTKVHTECC HGDLLECADD RADLAKYICE NQDSISSKLK ECCEKPLLEK SHCIAEVEND EMPADLPSLA ADFVESKDVC KNYAEAKDVF LGMFLYEYAR RHPDYSVVLL LRLAKTYETT LEKCCAAADP HECYAKVFDE FKPLVEEPQN LIKQNCELFE QLGEYKFQNA LLVRYTKKVP QVSTPTLVEV SRNLGKVGSK CCKHPEAKRM PCAEDYLSVV LNQLCVLHEK TPVSDRVTKC CTESLVNRRP CFSALEVDET YVPKEFNAET FTFHADICTL SEKERQIKKQ TALVELVKHK PKATKEQLKA VMDDFAAFVE KCCKADDKET CFAEEGKKLV AASQAALGL
Where the italicized first 24 amino acids are signal and propeptide portions not observed in the transcribed, translated and transported protein but present in the gene. There are 609 amino acids in this sequence with only 585 amino acids in the final product observed in the blood.
|This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Human_serum_albumin". A list of authors is available in Wikipedia.|