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Nile blue

Nile blue
IUPAC name Nile blue
Other names Nile blue A
CAS number 3625-57-8
PubChem 16938
SMILES CCN(CC)C1=CC2=C(C=C1)N=C3C4= CC=CC=C4C(=[NH2+])C=C3O2.[Cl-]
Molecular formula C20H20ClN3O
Molar mass 353.845 g/mol
Except where noted otherwise, data are given for
materials in their standard state
(at 25 °C, 100 kPa)

Infobox disclaimer and references

Nile blue (or Nile blue A) is a stain used in biology and histology. It may be used with live or fixed cells, and imparts a blue colour to cell nuclei.

It may also be used in conjunction with fluorescence microscopy to stain for the presence of polyhydroxybutyrate granules in prokaryotic or eukaryotic cells. Boiling a solution of Nile blue with sulfuric acid produces Nile red (Nile blue oxazone).

This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Nile_blue". A list of authors is available in Wikipedia.
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