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Nile red (also known as Nile blue oxazone) is a lipophilic stain. It is produced by boiling a solution of Nile blue with sulfuric acid. As can be seen from the structural formulae, this process replaces an amino group with a carbonyl group. Nile red stains intracellular lipid droplets red. Nile red is also intensely fluorescent, with a strong yellow-gold emission when in a lipid-rich environment.
Since the reaction to generate Nile red does not usually completely exhaust the supply of Nile blue, additional separation steps are required if pure Nile red is needed.
|This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Nile_red". A list of authors is available in Wikipedia.|