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Acridine orange is a nucleic acid selective fluorescent cationic dye useful for cell cycle determination. It is cell-permeable, and interacts with DNA and RNA by intercalation or electrostatic attractions. When bound to DNA, it is very similar spectrally to fluorescein, with an excitation maximum at 502 nm and an emission maximum at 525 nm (green). When it associates with RNA, the excitation maximum shifts to 460 nm (blue) and the emission maximum shifts to 650 nm (red). The dye is often used in epifluorescence microscopy.
Additional recommended knowledge
Acridine orange can be used in conjunction with ethidium bromide to differentiate between live and apoptotic cells.
|This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Acridine_orange". A list of authors is available in Wikipedia.|