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Fast atom bombardment

Fast atom bombardment mass spectrometry

ThermoQuest AvantGarde MS with quadropule detector and FAB/EI source.
Acronym FAB
Classification Mass spectrometry
Other Techniques
Related Secondary ion mass spectrometry
Matrix-assisted laser desorption/ionization
Plasma desorption mass spectrometry

Fast atom bombardment (FAB) is an ionization technique used in mass spectrometry.[1][2][3][4] The material to be analyzed is mixed with a non-volatile chemical protection environment called a matrix and is bombarded under vacuum with a high energy (4000 to 10,000 electron volts) beam of atoms. The atoms are typically from an inert gas such as argon or xenon. Common matricies include glycerol, thioglycerol, 3-nitrobenzyl alcohol (3-NBA), 18-Crown-6 ether, 2-nitrophenyloctyl ether, sulfolane, diethanolamine, and triethanolamine. This technique is similar to secondary ion mass spectrometry and plasma desorption mass spectrometry.


How it works

FAB is a relatively soft ionization technique and produces primarily intact protonated molecules denoted as [M+H]+ and deprotonated molecules such as [M-H]-. The nature of its ionization products places it close to electrospray and MALDI.[5]


  1. ^ Morris HR, Panico M, Barber M, Bordoli RS, Sedgwick RD, Tyler A (1981). "Fast atom bombardment: a new mass spectrometric method for peptide sequence analysis". Biochem. Biophys. Res. Commun. 101 (2): 623-31. PMID 7306100.
  2. ^ Barber, M.; Bordoli, R.S.; Sedgewick, R.D.; Tyler, A.N., Nature, 293, 1981, pp270-275
  3. ^ Barber, M.; Bordoli, R.S.; Elliott, G.J.; Sedgewick, R.D.; Tyler, A.N., Analytical Chemistry, 54, 1982, pp645A-657A.
  4. ^ Barber M, Bordoli RS, Sedgewick RD Tyler AN. "Fast atom bombardment of solids (F.A.B.): a new ion source for mass spectrometry". Journal of the Chemical Society, Chemical Communications: 325-7 year=1981.
  5. ^ Tomer KB. "The development of fast atom bombardment combined with tandem mass spectrometry for the determination of biomolecules". Mass Spectrometry Reviews 8: 445-82 year=1989.


  • Caprioli, R. M. (1990). Continuous-flow fast atom bombardment mass spectrometry. New York: Wiley. ISBN 0-471-92863-1. 

See also

This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Fast_atom_bombardment". A list of authors is available in Wikipedia.
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