Bronidox

  Bronidox® (Registered Tradmark of Cognis Corp) is chemical compound 5-Bromo-5-Nitro-1,3-Dioxane. Its use on goods without license from Cognis is strictly prohibited.

• Formula: C₄H₆BrNO₄
• Synonyms:
  • 5-Brom-5-Nitro-1,3-Dioxan
  • 5-Bromo-5-nitro-m-Dioxan or 5-Bromo-5-nitro-m-Dioxane
  • 1,3-Dioxane, 5-Bromo-5-Nitro-
  • BRONIDOX® L5
  • BRONIDOX® L
• Molecular weight: 212.06.

• Color:
  • white crystalline powder
  • colourless, transparent liquid in 1,2-propylene glycol
• Melting point: 60 °C [1] or 58.5-62°C [2]
• Light sensitive.
• Solubility: insoluble in water
• pH: 6-7
• Stable under normal conditions
• Non reactive with enzyme/substrate in assays but causes inhibition of enzyme activity n Bacteria [3]
• BRONIDOX® L5 is corrosive on metals [4]
•  ??? Incompabilities: strong oxidizing agents ??? [5]

• CAS Number: 30007-47-7
• EINECS Number: 250-001-7

Additional recommended knowledge

How to ensure accurate weighing results every day?

How to quickly check pipettes?

Daily Visual Balance Check

Used as

• Stabilizer
• Surfactant
• It is used in immunology for preserving antibodies and antisera in 0.1 - 0.5% concentration. It is used as preservative to avoid use of sodium azide.
• Bactericide. Very effective against yeast and fungi.
• used in cosmetics since the mid-1970s as preservative for shampoos, foam bath, etc. Maximum concentration is 0.1 %.

Safety

• Harmful
• Harmful if swallowed R22
• Toxic - tremor, convulsions, excitement
• Irritating to skin R38 - dermatitis
• wear suitable protective clothing S36
• relatively non-toxic to mammals
• some users do not recommend use in preparations destined for in vivo or tissue culture work [6]

• Combustion products: carbon monoxide, carbon dioxide, nitrogen oxides, hydrogen bromide gas, sulphur oxides, hydrogen chloride gas [7]

Toxicology

according to FSASAX

• Oral:
  • LD_{50, mouse, oral} 590 mg/kg 24 hours
  • LD_{50, rat, oral} 455 mg/kg 24 hours

• • LD_{50, rat, ipr.} 31 mg/kg 24 hours

• Skin:
  • LD_{50, mouse, skin} 2500 µg/24 hours
  • LD_{50, rat, skin} 2500 µg/24 hours

References

• http://www.chemicon.com/webfiles/MSDS/ECM711msds.pdf (Safety data sheet and first aid)

• http://www.chemicalland21.com/lifescience/phar/5-BROMO-5-NITRO-1,3-DIOXANE.htm
• http://www.fitzchem.com/mfg_cogniscare.shtml
• http://www.totalreproduction.com/products/specs/Bronidox.html
• Mode of action of the antimicrobial compound 5-bromo-5-nitro-1,3-dioxane (bronidox)
• http://www.labodia.com/en/intrinsic_factor/material_safety_data_sheet.pdf

See also

• Bronopol = 2-Bromo-2-nitropropane-1,3-diol