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Congo red is the sodium salt of benzidinediazo-bis-1-naphtylamine-4-sulfonic acid (formula: C32H22N6Na2O6S2; molecular weight: 696.66 g/mol). It is a secondary diazo dye. Congo red is water soluble, yielding a red colloidal solution; its solubility is better in organic solvents such as ethanol.
It has a strong, though apparently non-covalent affinity to cellulose fibres. However, the use of congo red in the cellulose industries (cotton textile, wood pulp & paper) has long been abandoned, primarily because of its tendency to change color when touched by sweaty fingers, to run and because of its toxicity.
Additional recommended knowledge
Congo red was first synthesized in 1883 by Paul Bottiger who was working then for the Friedrich Bayer Company in Elberfeld, Germany. He was looking for textile dyes that did not require a mordant step. The company was not interested in this bright red color, so he filed the patent under his name and sold it to the AGFA company of Berlin. AGFA marketed the dye under the name "Congo red", a catchy name in Germany at the time of the 1884 Berlin West Africa Conference, an important event in the Colonisation of Africa. The dye was a major commercial success for AGFA. In the following years, for the same reasons, other dyes were marketed using the "Congo" name: Congo rubine, Congo corinth, brilliant Congo, Congo orange, Congo brown, and Congo blue. 
Behaviour in solution
Due to a color change from blue to red at pH 3.0-5.2, congo red can be used as a pH indicator. Since this color change is an approximate inverse of that of litmus, it can be used with litmus paper in a simple parlor trick: add a drop or two of congo red to both an acid solution and a base solution. Dipping red litmus paper in the red solution will turn it blue, while dipping blue litmus paper in the blue solution will turn it red.
Congo red has a propensity to aggregate in aqueous and organic solutions. The proposed mechanisms suggest hydrophobic interactions between the aromatic rings of the dye molecules, leading to a pi-pi stacking phenomenon. Although these aggregates are present under various sizes and shapes, the "ribbon-like micelles" of a few molecules seem to be the predominant form (even if the "micelle" term is not totally appropriate here). This aggregation phenomenon is more important for high congo red concentrations, at high salinity and/or low pH.
As suggested by its intense red color, congo red has important spectrophotometric properties. Indeed, its UV-visible absorption spectrum shows a characteristic, intense peak around 498 nm in aqueous solution, at low dye concentration. Congo red molar extinction coefficient is about 45000 [L]/[mol].[cm] in these conditions. Aggregation of the dye tends to red-shift the absorption spectrum, whereas binding to cellulose fibres or amyloid fibrils has the opposite effect. Congo red also shows a fluorescent activity when bound to amyloid fibrils, which tends to be used as a sensitive diagnosis tool for amyloidosis, instead of the traditional histological birefringence test.
In biochemistry and histology, congo red is used to stain microscopic preparates, especially as a cytoplasm and erythrocyte stain. Apple-green birefringence of Congo red stained preparates under polarized light is indicative for the presence of amyloid fibrils.
|This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Congo_red". A list of authors is available in Wikipedia.|