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Additional recommended knowledge
Inhibition of translation
Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that causes premature chain termination during translation taking place in the ribosome. Part of the molecule resembles the 3' end of the aminoacylated tRNA. It will enter the A site and transfer to the growing chain, causing premature chain release. The exact mechanism of action is unknown at this time, but, the 3' position contains an amide linkage instead of the normal ester linkage of tRNA, the amide bond makes the molecule much more resistant to hydrolysis and thus causes the ribosome to become stopped.
It is not selective for either prokaryotes or eukaryotes.
Also of note, puromycin is critical in mRNA display as it allows the growing peptide chain to be covalently bonded to its own mRNA template.
|This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Puromycin". A list of authors is available in Wikipedia.|