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Shotgun proteomics

Shotgun proteomics is a method of identifying proteins in complex mixtures using a combination of high performance liquid chromatography combined with mass spectrometry.[1][2][3] The name is derived from shotgun sequencing of DNA which is itself named by analogy with the rapidly-expanding, quasi-random firing pattern of a shotgun. In shotgun proteomics, the proteins in the mixture are digested and the resulting peptides are separated by liquid chromatography. Tandem mass spectrometry is then used to identify the peptides.

See also


  1. ^ Hu L, Ye M, Jiang X, Feng S, Zou H (2007). "Advances in hyphenated analytical techniques for shotgun proteome and peptidome analysis--a review". Anal. Chim. Acta 598 (2): 193–204. doi:10.1016/j.aca.2007.07.046. PMID 17719892.
  2. ^ Fournier ML, Gilmore JM, Martin-Brown SA, Washburn MP (2007). "Multidimensional separations-based shotgun proteomics". Chem. Rev. 107 (8): 3654–86. doi:10.1021/cr068279a. PMID 17649983.
  3. ^ Nesvizhskii AI (2007). "Protein identification by tandem mass spectrometry and sequence database searching". Methods Mol. Biol. 367: 87–119. doi:10.1385/1-59745-275-0:87. PMID 17185772.


  • Marcotte EM (2007). "How do shotgun proteomics algorithms identify proteins?". Nat. Biotechnol. 25 (7): 755–7. doi:10.1038/nbt0707-755. PMID 17621303.
  • Yates, John W.; Veenstra, Timothy D.. Proteomics for Biological Discovery. New York: Wiley-Liss. ISBN 0-471-16005-9. 
This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Shotgun_proteomics". A list of authors is available in Wikipedia.
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