1,353 rat liver genes are spotted onto the array using MWG-Biotech’s
1gene/1oligo method. Every bioinformatoricly designed HPSF®
oligonucleotide is 100% gene specific and thus guarantees the
identification of the respective gene.
In the development of this array MWG-Biotech’s Genomic Discovery
concept has been applied on all stages from sequencing via
bioinformatics through to microarray development. MWG-Biotech’s own
sequencing department identified 1,166 genes from tissue samples of
12 week old rats. Additionally, 187 new genes were found that up to now
had not been known in the rat. These genes or rat liver transcripts
(mRNAs that are translated into proteins) were identified by means of
comparative analysis with human and mouse genes.
88 of these 187 genes were derived from stroke-prone spontaneously
hypertensive (with high blood pressure) rats (SHRSP), 65 from
normotensive (with normal blood pressure) rats, the other 34 genes
could be found in both strains.
The Pan® Rat Liver Array is used for expression studies of the rat liver
to determine which genes are switched on or off in a specific process or
by a specific substance. This makes the array an important
pharmaceutical tool for testing drug toxicity, the so-called fields of
The use of the Pan® Rat Liver Array will sharply reduce the amount of
animal testing needed. By means of the liver signature genes spotted
onto the array it is possible to determine which genes are regulated in
which ways by certain toxic substances. With this the suitability of a
specific chemical compound for further drug development can be
assessed. To safeguard the same degree of accuracy as gained from
the array experiment a large amount of animal experiments would be
required. The high number of parallel processes that can be performed
in one single microarray experiment also leads to considerable time and
As MWG-Biotech’s Pan® Rat Liver Array also contains genes connected
to hypertension it is of importance for the research of cardiovascular
diseases in humans. It will also play an important role in the investigation
of important metabolic pathways.