Wavelength Accuracy in UV/VIS Spectrophotometry

Wavelength accuracy and repeatability are key performance aspects of UV/VIS instruments. An accurate calibration of the wavelength axis is crucial for every application relying on a spectroscopic technique and is the basis for its use as a reliable analytical tool.

This white paper discusses different theoretical and practical wavelength aspects of UV/VIS spectrophotometers in three main sections:

• Requirements for wavelength accuracy, range and repeatability in the EU, UK, US, CN and JP Pharmacopoeias
• Examples of different applications and ASTM standards are outlined
• Theoretical and practical details on wavelength calibrations and adjustment procedures and standards are provided