My watch list
my.chemeurope.com  
Login  

Protein methods



Protein methods are the techniques used to study proteins.

Additional recommended knowledge

Contents

List of Protein Methods

There are genetic methods for studying proteins, methods for detecting proteins, methods for isolating and purifying proteins and other methods for characterizing the structure and function of proteins, often requiring that the protein first be purified.

Genetic methods

  • conceptual translation- many proteins are never directly sequenced, but their sequence of amino acids is known by "conceptual translation" of a known mRNA sequence. See Genetic code.
  • site-directed mutagenesis allows new variants of proteins to be produced and tested for how structural changes alter protein function.
  • evolutionary; analysis of sequence changes in different species using software such as BLAST.
  • Proteins that are involved in human diseases can be identified by matching alleles to disease and other phenotypes using methods such as calculation of LOD scores.

Detecting proteins

Protein purification

  • Protein Isolation
    • chromatography methods
  • Protein Extraction and Solubilization
  • Protein Concentration Determination Methods, Bradford protein assay
  • Concentrating Protein Solutions
  • Gel electrophoresis
    • Gel Electrophoresis Under denaturing conditions
    • Gel Electrophoresis Under non-denaturing conditions
    • 2D Gel Electrophoresis
  • Electrofocusing

Protein structures

Other methods

References

  • "Protein Methods", 2nd Edition by Daniel M. Bollag, Michael D. Rozycki and Stuart J. Edelstein (1996) Published by Wiley Pblishers ISBN 0-471-11837-0.
 
This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Protein_methods". A list of authors is available in Wikipedia.
Your browser is not current. Microsoft Internet Explorer 6.0 does not support some functions on Chemie.DE