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Protein-protein docking is the determination of the molecular structure of complexes formed by two or more proteins without the need for experimental measurement. The study of protein-protein docking was boosted by the rapid increase in available protein structures of the 1990s, and it has now been under intensive research for over a decade. Many proteins which remain relatively rigid upon complexation can now be successfully docked. Methods are under development to handle cases where the internal conformation of one or more of the partners changes substantially.
Protein-protein docking generally does not refer to describing the path taken by the components during complexation; the only object of docking is the final complexed state. Since the natural use of "docking" suggests guidance along a path, "protein-protein docking" may be regarded as a misnomer.
Additional recommended knowledge
For most of the proteins known to science, their biological role, as characterized by which other proteins they interact with, is incompletely understood. Even proteins which participate in a well-understood biological process (e. g. the Krebs cycle) may have interaction partners or functions which are unrelated to that process. Moreover, vast numbers of "hypothetical" proteins were discovered in the genomic revolution of the late 1990's, about which there remains no information at all, apart from their amino acid sequence.
In cases of known protein-protein interactions, other questions arise. Genetic diseases are known to be caused by misfolded or mutated proteins (e. g. cystic fibrosis), and there is a desire to understand what, if any, anomalous protein-protein interactions a given mutation can cause. In the distant future, proteins may be designed to perform biological functions, and a determination of the potential interactions of such proteins will be essential.
For any given set of proteins, the following questions may arise:
Protein-protein docking is proposed to have the ultimate potential to address all these issues comprehensively. Furthermore, since docking methods can be based on purely physical principles, even proteins of unknown function (or which have been studied relatively little) may be docked. The only prerequisite is that their molecular structure has been either determined experimentally, or can be estimated by some theoretical technique (see protein structure prediction).
Rigid-body docking vs. flexible docking
If the bond angles, bond lengths and torsion angles of the components are not modified at any stage of complex generation, it is known as rigid body docking. A subject of speculation is whether or not rigid-body docking is sufficiently good for most docking. When substantial conformational change occurs within the components at the time of complex formation, rigid-body docking is inadequate. However, scoring all possible conformational changes is prohibitively expensive in computer time. Docking procedures which permit conformational change, or flexible docking procedures, must intelligently select small subset of possible conformational changes for consideration.
Successful docking requires two criteria:
For many interactions, the binding site is known on one or more of the proteins to be docked. This is the case for antibodies and for competitive inhibitors. In other cases, a binding site may be strongly suggested by mutagenic or phylogenetic evidence. Configurations where the proteins interpenetrate severely may also be ruled out a priori.
After making exclusions based on prior knowledge or stereochemical clash, the remaining space of possible complexed structures must be sampled exhaustively, evenly and with a sufficient coverage to guarantee a near hit. Each configuration must be scored with a measure that is capable of ranking a nearly correct structure above at least 100,000 alternatives. This is a computationally intensive task, and a variety of strategies have been developed.
Reciprocal space methods
Each of the proteins may be represented as a simple cubic lattice. Then, for the class of scores which are discrete convolutions, configurations related to each other by translation of one protein by an exact lattice vector can all be scored almost simultaneously by applying the convolution theorem. It is possible to construct reasonable, if approximate, convolution-like scoring functions representing both stereochemical and electrostatic fitness.
Reciprocal space methods have been used extensively for their ability to evaluate enormous numbers of configurations. They lose their speed advantage if torsional changes are introduced. Another drawback is that it is impossible to make efficient use of prior knowledge. The question also remains whether convolutions are too limited a class of scoring function to identify the best complex reliably.
Monte Carlo methods
In Monte Carlo, an initial configuration is refined by taking random steps which are accepted or rejected based on their induced improvement in score (see the Metropolis criterion), until a certain number of steps have been tried. The assumption is that convergence to the best structure should occur from a large class of initial configurations, only one of which needs to be considered. Initial configurations may be sampled coarsely, and much computation time can be saved. Because of the difficulty of finding a scoring function which is both highly discriminating for the correct configuration and also converges to the correct configuration from a distance, the use of two levels of refinement, with different scoring functions, has been proposed. Torsion can be introduced naturally to Monte Carlo as an additional property of each random move.
Monte Carlo methods are not guaranteed to search exhaustively, so that the best configuration may be missed even using a scoring function which would in theory identify it. How severe a problem this is for docking has not been firmly established.
Selecting the docked complex structure
To find a score which forms a consistent basis for selecting the best configuration, studies are carried out on a standard benchmark (see below) of protein-protein interaction cases. Scoring functions are assessed on the rank they assign to the best structure (ideally the best structure should be ranked 1), and on their coverage (the proportion of the benchmark cases for which they achieve an acceptable result). Types of scores studied include:
It is usual to create hybrid scores by combining one or more categories above in a weighted sum whose weights are optimized on cases from the benchmark. To avoid bias, the benchmark cases used to optimize the weights must not overlap with the cases used to make the final test of the score.
A benchmark of 84 protein-protein interactions with known complexed structures has been developed for testing docking methods. The set is chosen to cover a wide range of interaction types, and to avoid repeated features, such as the profile of interactors' structural families according to the SCOP database. Benchmark elements are classified into three levels of difficulty (the most difficult containing the largest change in backbone conformation). The protein-protein docking benchmark contains examples of enzyme-inhibitor, antigen-antibody and homomultimeric complexes.
The CAPRI assessment
The Critical Assessment of Predicted Interactions (CAPRI) is an ongoing series of events in which researchers throughout the community try to dock the same proteins, as provided by the assessors. Rounds take place approximately every 6 months. Each round contains between one and six target protein-protein complexes whose structures have been recently determined experimentally. The coordinates and are held privately by the assessors, with the cooperation of the structural biologists who determined them. The assessment of submissions is double blind.
CAPRI attracts a high level of participation (37 groups participated worldwide in round seven) and a high level of interest from the biological community in general. Although CAPRI results are of little statistical significance owing to the small number of targets in each round, the role of CAPRI in stimulating discourse is significant. (The CASP assessment is a similar exercise in the field of protein structure prediction).
Deciding whether a complex actually occurs in nature and measuring its affinity
A reliable method for affinity prediction has the potential to transform biochemistry and cell biology. Though a distant prospect, affinity prediction may be considered the as the ultimate achievement in protein-protein docking.
Protein-protein docking and molecular docking
The field of protein-protein docking is highly computationally oriented, and it shares approaches with molecular docking. Molecular docking is sometimes referred to as small-molecule docking, to distinguish it from protein-protein docking. Proteins complexed with polynucleotide molecules are widely studied using similar or identical approaches to protein-protein docking, although if the nucleotide molecule is small enough, the case may be framed as a molecular docking problem.
|This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Protein-protein_docking". A list of authors is available in Wikipedia.|