i3 Digital Membrane Chromatography

Digital Membrane Chromatography - Gentle Protein Purification at the Push of a Button:

The purification of biological therapeutics is one of the critical steps in biopharmaceutical research and process development. It is indispensable, particularly in the analysis and production of modern therapeutics for antibody-based gene therapies. Membrane chromatography has become established as a powerful and efficient method for this purpose. Until now, however, the use of acid or other chemicals has been required to purify valuable therapeutics by chromatography. With digital membrane chromatography, or DMC, i3 Membrane is opening up a new path in protein purification. Instead of chemically eluting bound molecules, DMC uses a precisely controlled electrical potential. This enables target molecules to be released from the membrane surface particularly quickly and gently without conventional acidic elution conditions and without additional buffer exchange.The result: highly concentrated, ready-to-use eluates that can be used directly for analytical testing, screening workflows, or subsequent process steps.

For the purification of mAbs from Protein A, i3 Membrane combines the advantages of membrane chromatography with a digitally controlled elution process. The i3 DMC PrA Membrane Adsorber is equipped with a Protein A membrane and was specifically developed for the rapid purification of antibodies and Fc-containing proteins.The electrical potential is applied directly to the membrane via an ultrathin conductive gold layer. Four integrated contact elements enable seamless connection to the i3 DMC Control Smart Control Unit. This allows the elution process to be controlled precisely, reproducibly, and easily. Because no additional buffer exchange is required, the workflow becomes significantly more efficient. At the same time, eliminating low-pH elution buffers reduces the risk of aggregation while improving yield.

DMC was developed for applications where speed, product protection, and analytical relevance are essential. Especially in early development phases, screening, and the characterization of sensitive biomolecules, the technology creates new flexibility.Researchers benefit from a compact, easily integrated system that can be incorporated into existing liquid chromatography workflows. The purified eluates are immediately available for downstream analyses—from protein analytics to method and process development.

• Gentle Elution - Elution via electrical potential instead of conventional acidic conditions.
• Reduced Risk of Aggregation - More stable conditions for sensitive biomolecules.
• Fewer Process Steps - No additional buffer exchange required.
• Highly Concentrated Eluates in Neutral Buffers - Ready for direct use in analytical testing and development workflows.
• Easy integration - Compatible with existing liquid chromatography setups.

With digital membrane chromatography, i3 Membrane is setting a new milestone in protein purification. Contact us for more information, including information on your specific applications.