How graphene nanoparticles improve the resolution of microscopes
Small particles, big effects
Microscopy is an important investigation method, in physics, biology, medicine, and many other sciences. However, it has one disadvantage: its resolution is limited by physical principles. Structures can only be imaged if they are separated by a distance greater than half the wavelength of light. With blue light, this corresponds to a distance of approximately 200 nanometers, i.e., 200 millionths of a millimeter.
This limit can be circumvented using superresolution microscopy. Today there are a number of different such approaches. In the type of superresolution microscopy applied here, fluorescent particles are excited by light and re-emit light at a slightly different wavelength, i.e. a slightly different color. The position of these fluorescent particles can be determined with higher precision than given by the wavelength of the light: If they are blinking randomly, two neighbouring particles typically do not light up simultaneously – this means that their signals do not overlap and thus the positions of the individual particles can be determined independently of each other, so that even at very small distances the particles can be imaged separately, i.e. "resolved". Researchers at the MPI-P have now shown that nanoparticles from graphene – so-called nanographenes, consisting of a carbon layer only one atom thick - have properties that are ideal for this special microscopy technique.
In the past, several fluorescent materials have been used for this type of microscopy, including dyes, so-called quantum dots, and fluorescent proteins. Nanographenes are as good as the best of these materials in terms of their optical properties. In addition to their excellent optical properties, nanographenes are non-toxic, very small, and, most notably and different from all other materials, their flashing frequency is robust and independent of their environment. This means that nanographene can be used in air, aqueous solutions and other solvents - making it a very versatile fluorophore. Nanographenes can further be readily modified so that they only adhere to certain interesting locations on a sample, e.g., a specific organelle in a cell.
"We have compared nanographene with the gold standard in this microscopy technique - the organic dye Alexa 647," says Prof. Mischa Bonn, director at the MPI-P. "We found that nanographene is as efficient as this dye, i.e., it can convert as much of the incident light into a different color, but doesn’t require a specifically tailored environment that Alexa requires.“
To test the nanographene produced at the Max Planck Institute for Polymer Research, the scientists collaborated with Prof. Christoph Cremer's group at the Institute of Molecular Biology (IMB) in Mainz. The researchers prepared a glass surface with nanometer-sized fissures. Here, nanographene particles were applied, which were mainly deposited in the gaps. In comparison with conventional microscopy, they were able to show that the resolution could be increased by a factor of 10 using graphene nanoparticles.
The scientists see the development of their material as an important step in superresolution microscopy.
Other news from the department science
Blue-green algae sugar instead of glyphosate
Cooperation project develops environmentally friendly glyphosate alternative
Using clay to combat eternal toxins
TU Freiberg clarifies basis for innovative PFAS filter made of clay
Unveiling a new era of imaging: Boston University engineers lead breakthrough microscopy techniques
Researchers made significant advancements in the field of vibrational imaging
Phasing out fossil fuels could save millions of lives
The mortality burden attributable to air pollution from fossil fuel use is considerably higher than most previous estimates - a phaseout of fossil fuels would have tremendous, positive health outcomes
Replicating the structure of bird feathers
The new material could be used in batteries or filtration
Quantum tool opens door to uncharted phenomena
Method can contribute to a better understanding of quantum materials
Recovering instead of shredding: recycling batteries more efficiently
KIT researchers are working with industry to develop a more sustainable recycling process to recycle materials from lithium-ion batteries more effectively
Industry 4.0: No impact on energy consumption?
To what extent does the digitalisation of industrial and manufacturing processes (Industry 4.0) improve energy efficiency and thus reduce energy intensity?
New approach to the sensible utilisation of carbon dioxide from car exhaust gases
"A method has been discovered that uses impure CO2 streams and enables a breakthrough in the synthesis of valuable chemicals and pharmaceuticals"
Boosting PET recycling with higher standards for laboratory experiments
New study shows how enzymatic plastic degradation could be brought one step closer to commercialisation
Innovating Optoelectronic Components with Phosphorus
Significant breakthrough: phosphorus chemists develop new method to selectively introduce phosphorus and nitrogen atoms into polyaromatic systems
Artificial intelligence finds ways to develop new drugs
The chemists tested the process using borylation – a reaction that activates hydrocarbon scaffolds
X-rays reveal how glasses lose their stability
PETRA III experiment shows how atoms in glass behave as weaknesses appear
Most read news
Plastic-eating bacteria turn waste into useful starting materials for other products
Microbial Upcycling of Waste PET
Microbes could help reduce the need for chemical fertilizers
A coating protects nitrogen-fixing bacteria: Start-up to commercialise coated bacteria for large-scale use in regenerative agriculture
New designs for solid-state electrolytes may soon revolutionize the battery industry
Scientists achieve monumental improvements in lithium-metal-chloride solid-state electrolytes
Dow and Evonik announce startup of hydrogen peroxide to propylene glycol (HPPG) pilot plant
Innovative technology offer flexibility, lower costs, and a smaller environmental footprint
Inauguration of the world’s first pilot plant for the cost-efficient production of green methanol
Start-up C1 Green Chemicals AG and research partners develop fundamentally new production process
This is a battery
Two colored liquids bubbling through tubes: Is this what the battery of the future looks like?
Converting PFAS “forever chemicals” into valuable compounds
Scientists develop a new method to incorporate harmful perfluoroalkenes into N-heterocyclic carbene ligands
Not so silver lining: Microplastics found in clouds could affect the weather
Low-altitude and denser clouds contained greater amounts of microplastics
Graphene's proton permeability: A switch for future energy technologies
This discovery could lead to the development of more efficient hydrogen fuel cells and solar water-splitting devices
Lithium-ion batteries are no longer the gold standard in battery tech
On the way to safer and more powerful energy sources
CO2-free hydrogen: BASF receives funding approval for 54-megawatt water electrolysis plant
Proton exchange membrane (PEM) electrolyzer expected to produce up to 8,000 metric tons of hydrogen per year
More news from our other portals
Bowel cancer: aspirin activates protective genes
Researchers have identified a signaling pathway by which aspirin can inhibit colorectal cancer.
Dunning-Kruger effect with muesli bars
Those who know the least consider themselves highly competent
Autonomous measuring instruments systematically detect new materials
A new algorithm measures materials libraries up to four times faster than before: It’s based on machine learning
New drug delivery system could reduce daily diabetes shots to just three a year
Dietary management drugs have transformed Type 2 diabetes care, but daily injection routines are challenging for some patients
Naked Clams: The New Superfood Sensation Emerging from the Depths
Researchers found Naked Clams contain almost twice the amount of Vitamin B12 as blue mussels and have developed an efficient way to farm them
Researchers discover new ultra strong material for microchip sensors
A material that doesn't just rival the strength of diamonds and graphene, but boasts a yield strength 10 times greater than Kevlar
Pushers, overcrowded trains and phone zombies
Sprite presents the world's first vending machine that responds to the things that bother Generation Z the most
Aston University technology to combat the not-so sweet practice of honey fraud
Light technology to be used to detect if honey is blended with cheap additions
How stem cells and immune cells communicate
Lisec Artz Award for Simon Haas: Groundbreaking discovery of an unknown protective mechanism against blood cancer from stem cells
Scientists use quantum biology, AI to sharpen genome editing tool
"This study represents an exciting advancement toward, understanding how we can avoid making costly ‘typos’ in an organism’s genetic code"
From the trough to the plate - digitally calculated
Computer program "ConTrans" estimates how much of an undesirable substance is transferred from animal feed to food
Viral Impostors: Breakthrough for Virus Research
The penetration of viruses into cells can now be tracked with unprecedented accuracy thanks to innovative design for pseudoviruses
Fatty acid factory filmed at work
High-resolution images provide new insights into cellular fatty acid production: Potential for medicine and biotechnology
Textbook knowledge turned on its head: 3-in-1 microorganism discovered
Newly multifunctional bacterial species
Tönnies Group launches first nationwide "Meat Climate Platform"
100 guests at the Future Forum for Agriculture
Tracking down Environmental Toxins
Detection of per- and polyfluoroalkyl substances (PFAS) by interrupted energy transfer
Could eating turkey ease colitis?
According to data in mice, extra tryptophan could reduce the risk of future colitis flares
The weight of pollution: exposure linked to obesity
Chronic exposure to environmental pollutants found to increase risk of cardiovascular disease
Are healthy foods automatically sustainable, too?
Perceptions about sustainability and healthy food choices are closely linked