Thermo Fisher Scientific Solution Successfully Detects Anabolic Androgenic Steroids in Horse Biological Fluids
Thermo Fisher Scientific Inc. announced the success of its liquid chromatography tandem mass spectrometry (LC-MS/MS) solution at the Kenneth L. Maddy analytical chemistry Laboratory. The Thermo Scientific turbulent flow chromatography system coupled with the Thermo Scientific triple stage quadrupole mass spectrometer was selected to perform precise and sensitive detection of anabolic androgenic steroids (AAS) in urine and plasma samples from performance horses. Based in Davis, California, the laboratory is the authorized equine drug-testing laboratory for California's six permanent race courses, nine seasonal fair venues and other performance events.
One of the major challenges when determining AAS in biological fluids is sample preparation. Endogenous phospholipids, present in extremely high levels in biological samples, are a major component of matrix interference. These interferences affect analyte ionization, reducing the precision and reproducibility of subsequent quantitative MS analysis (ion suppression). Equine testing laboratories have traditionally used immunoassay screening followed by gas chromatography/mass spectrometry (GC/MS) confirmation to minimize interferences. However, these methods lack sensitivity, require lengthy sample preparation and do not remove all matrix interferences.
The Kenneth L. Maddy Analytical Chemistry Laboratory required a means of analysis with high accuracy and simple sample preparation to cater for the vast number of incoming samples. Dr. Scott Stanley, Associate Professor of Analytical Chemistry and Pharmacology and head of the laboratory, decided to invest in a comprehensive Thermo Scientific solution. Researchers have been able to detect very small quantities (low parts per trillion) of substances in blood long after an illegal administration. The labor-intensive SPE and LLE procedures have been eliminated.
Dr. Stanley comments, "The system has streamlined our method development phase, eliminated lengthy and costly sample preparation steps, increased sample throughput and improved detection sensitivity and accuracy. In addition, the method limit of quantitation has improved five - ten fold over previous methods. The new system has become the cornerstone for our AAS research and regulatory testing program and I would estimate overall savings of several hundred thousand dollars. The initial return on investment will take approximately 18-20 months with the instrument life expectancy being five to seven years minimum."
Other news from the department research and development
Get the chemical industry in your inbox
From now on, don't miss a thing: Our newsletter for the chemical industry, analytics, lab technology and process engineering brings you up to date every Tuesday and Thursday. The latest industry news, product highlights and innovations - compact and easy to understand in your inbox. Researched by us so you don't have to.
Most read news
More news from our other portals
See the theme worlds for related content
Topic world Gas chromatography
Gas chromatography is an essential method in analytical chemistry for the separation and analysis of volatile compounds. Due to its high resolution and sensitivity, it has become firmly established in areas such as environmental analysis, food chemistry or forensic science. GC provides precise and reliable results and enables deep insights into the chemical composition of samples.
Topic world Gas chromatography
Gas chromatography is an essential method in analytical chemistry for the separation and analysis of volatile compounds. Due to its high resolution and sensitivity, it has become firmly established in areas such as environmental analysis, food chemistry or forensic science. GC provides precise and reliable results and enables deep insights into the chemical composition of samples.
Topic World Spectroscopy
Investigation with spectroscopy gives us unique insights into the composition and structure of materials. From UV-Vis spectroscopy to infrared and Raman spectroscopy to fluorescence and atomic absorption spectroscopy, spectroscopy offers us a wide range of analytical techniques to precisely characterize substances. Immerse yourself in the fascinating world of spectroscopy!
Topic World Spectroscopy
Investigation with spectroscopy gives us unique insights into the composition and structure of materials. From UV-Vis spectroscopy to infrared and Raman spectroscopy to fluorescence and atomic absorption spectroscopy, spectroscopy offers us a wide range of analytical techniques to precisely characterize substances. Immerse yourself in the fascinating world of spectroscopy!
Topic World Chromatography
Chromatography enables us to separate, identify and thus understand complex substances. Whether in the food industry, pharmaceutical research or environmental analysis - chromatography opens up a treasure trove of information about the composition and quality of our samples. Discover the fascinating world of chromatography!
Topic World Chromatography
Chromatography enables us to separate, identify and thus understand complex substances. Whether in the food industry, pharmaceutical research or environmental analysis - chromatography opens up a treasure trove of information about the composition and quality of our samples. Discover the fascinating world of chromatography!
Topic World Mass Spectrometry
Mass spectrometry enables us to detect and identify molecules and reveal their structure. Whether in chemistry, biochemistry or forensics - mass spectrometry opens up unexpected insights into the composition of our world. Immerse yourself in the fascinating world of mass spectrometry!
Topic World Mass Spectrometry
Mass spectrometry enables us to detect and identify molecules and reveal their structure. Whether in chemistry, biochemistry or forensics - mass spectrometry opens up unexpected insights into the composition of our world. Immerse yourself in the fascinating world of mass spectrometry!
